Efectos tóxicos de la sacarosa e ion amonio, en larvas de rana Osteopilus septentrionalis (Anura: Ranidae)n°12 [mar-may 2007] Retel
Trujillo Hernández. J, Carballo Hondal. O, Castañeda León. J.M, Álvarez Pérez. E, Reyes González A. Centro Nacional de Toxicología
Palabras claves: sacarosa, ion amonio, larvas de rana,
toxicidad, Osteopilus septentrionalis
Toxic Effects of Saccharosa and Ammonium Ion in Osteopilus
The culture media used in the production of bioproducts for agricultural purposes are rich in carbon hydrates. There are some organisms unable to assimilate these carbon hydrates. Formulations containing ammonium ions are potentially toxic. The objectives of this work are to record sings of toxicity or mortality in larvae of frogs (Osteospilus septentrionalis), macroscopic and histological damages in organs and tissues of larvae exposed to the culture medium and to ammonium sulphate. A static 96 hours study was carried out with healthy larvae, between the 25 – 26 stagets according to Gosner classification. The concentrations evaluated were: 0.1, 1.0, 10.0 and 100.0 mg/l for both culture medium and Ammonium Sulphate. Concentrations were calculated according to their proportion in the final formulation. Only under the 100.0 mg/l concentration, the 100 % of larvae died after 96 hours. Both groups showed signs of toxicity, evidenced by injury in organs and tissues (liver, vesicle, pancreas and intestines). An excessive ingestion of carbon hydrates (saccharosa) caused cell degeneration in liver and pancreas cells, this finding coincided with the reports of other authors. It is concluded that the excessive ingestion of saccharosa and ammonium caused death and damages to the frog larvae Osteospilus septentrionalis kept under pond conditions.
Keywords: saccharosa, ammonium ions, larvae or frogs,
toxicity, Osteopilus septentrionalis