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The Tox21 10 K chemical profiles for in vivo toxicity prediction and mechanism characterization

26 January, 2016
Cell-based tests promise respite for lab animals: study. phys.org, January 26, 2016. Scientists in the United States said Tuesday they were developing a faster, more efficient way of gauging the toxicity of chemicals, which may reduce the need for animal testing.Using human cells in Petri dishes in the lab, they tested about 10,000 different types of chemical compounds including pesticides, industrial chemicals, food additives and drugs. The results were used to build models to "predict" whether the compounds, or combinations of them, may be harmful to humans or the environment when used in new drugs or environmental chemicals.
The Tox21 10 K chemical profiles for in vivo toxicity prediction and mechanism characterization
Monos en Costa Rica, , bien lejos de la experimentación animal
Toxicity is one of the main reasons that new drugs fail, and it is hoped this library of toxicity data may spot unsafe chemical compounds at a far earlier phase of research.
"Thousands of chemicals to which humans are exposed have inadequate data on which to predict their potential for toxicological effects," the study authors wrote in the journal Nature Communications.
Traditional toxicity tests using animals are expensive and species differences mean they do not always accurately predict a chemical’s effect on humans.
They also raises ethical concerns about animal welfare.
The project, dubbed Tox21, is a joint effort involving three US federal agencies: the Environmental Protection Agency, the National Institutes of Health (NIH) and the Food and Drug Administration (FDA).
"An important goal of the US Tox21 program is to use in vitro (Petri dish) data as surrogates for in vivo (live) toxicity to reduce animal testing," study co-author Ruili Huang of the NIH told AFP by email.
But to achieve this, cell tests have to be just as predictive, or more so, of human toxicity as animal tests.
After testing about 10,000 compounds, each in 15 different concentrations and on different cells, the team used their database to build predictive models for new chemical combinations.
They found that their models could predict toxicity for both humans and animals, and may be "a promising alternative to traditional animal toxicology studies."
Further work must be done to validate and improve the models, which Huang said may never completely replace animal testing.
It may, however, allow scientists to prioritise chemicals predicted to be most likely toxic for further tests using traditional methods, and so "significantly reduce the amount of animal tests required," he said.Animal activists have welcomed the research.
"To understand more about human development and human diseases, the world’s most forward-thinking scientists are developing and implementing methods that supersede the crude use of animals," Julia Baines, science policy advisor of the organisation People for the Ethical Treatment of Animals (PETA) told AFP.
"PETA welcomes the wealth of advanced non-animal research methodologies that are creating a brighter future for animals and human health."
More information: Modelling the Tox21 10 K chemical profiles for in vivo toxicity prediction and mechanism characterization, Nature Communications 7, Article number: 10425 DOI: 10.1038/ncomms10425


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